Figure 3: Positive selection of IL2-KHYG1 cells in the absence of IL2. (a) FACS profile of IL2-KHYG1 cells was determined at the indicated time points after culturing cells with medium having no IL2. (b) FACS profile shows the % of YFP⁺ cells 83 days after the withdrawal of IL2.This is the time point YFP⁺ cells were sorted post-transduction of KHYG1 with IL2. (c) Comparison of the % of YFP⁺ cells at different time points after withdrawal of IL2. Data are mean ± SD of two independent experiments