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Figure 2: Positive selection of IL2-KHYG1 cells with limiting doses of IL2 concentrations. The FACS profile showing the % of YFP+ cells of the IL2 transduced KHYG1 cells after culturing cells with progressively decreasing doses of IL2 for 7 (A, left) ,14 (B, left) and 28 days (C, left). Comparison of the % of YFP+ cells in empty vector or IL2 transduced KHYG1 cells 7 (A, right), 14 (B, right) or 28 (C, right) days after culturing cells with progressively decreasing doses of IL2. Data are mean ± SD of two independent experiments |
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