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  Indian J Med Microbiol
 

Figure 1: Transduction of KHYG1 cells with IL2 and cell culture in limiting doses of IL2. (a) The IL2-PMIY retroviral construct generated for ectopic IL2 expression. (b) The FACS profile showing the % of YFP+ cells of the empty vector or IL2 transduced KHYG1 cells after culturing with progressively decreasing doses of IL2 for 7 days. (c) Quantification of the % of YFP+ cells in vector or IL2 transduced KHYG1 cells 7 days after culturing cells with progressively decreasing doses of IL2. EV: Empty vector

Figure 1: Transduction of KHYG1 cells with IL2 and cell culture in limiting doses of IL2. (a) The IL2-PMIY retroviral construct generated for ectopic IL2 expression. (b) The FACS profile showing the % of YFP<sup>+</sup> cells of the empty vector or IL2 transduced KHYG1 cells after culturing with progressively decreasing doses of IL2 for 7 days. (c) Quantification of the % of YFP<sup>+</sup> cells in vector or IL2 transduced KHYG1 cells 7 days after culturing cells with progressively decreasing doses of IL2. EV: Empty vector