Figure 1: Transduction of KHYG1 cells with IL2 and cell culture in limiting doses of IL2. (a) The IL2-PMIY retroviral construct generated for ectopic IL2 expression. (b) The FACS profile showing the % of YFP⁺ cells of the empty vector or IL2 transduced KHYG1 cells after culturing with progressively decreasing doses of IL2 for 7 days. (c) Quantification of the % of YFP⁺ cells in vector or IL2 transduced KHYG1 cells 7 days after culturing cells with progressively decreasing doses of IL2. EV: Empty vector