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  Indian J Med Microbiol
 

Figure 5: Western blotting analysis of expressed recombinant lysostaphin using antiHis-tagantibody. Lanes1, protein molecular size marker (Sigma Aldrich, ColorBurst™); 2, W Bnegative control (uninduced recombinant E. coli BL21); 3 and 4, recombinant E. coli BL21 induced by 4 h after induction with 0.5 m MIPTG; 5 and 6, E. coli BL21 cell lysate transformed by recombinant plasmid 4 h after induction with1m MIPTG

Figure 5: Western blotting analysis of expressed recombinant lysostaphin using antiHis-tagantibody. Lanes1, protein molecular size marker (Sigma Aldrich, ColorBurst™); 2, W Bnegative control (uninduced recombinant <i>E. coli</i> BL21); 3 and 4, recombinant <i>E. coli</i> BL21 induced by 4 h after induction with 0.5 m MIPTG; 5 and 6, <i>E. coli</i> BL21 cell lysate transformed by recombinant plasmid 4 h after induction with1m MIPTG