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  Indian J Med Microbiol
 

Figure 4: GeneXpert MTB/RIF Assay Procedure [Ref. Boehme et al , NEJM 2010][62] Add two volumes of sample treatment reagent to each volume of sputum. Mix well, incubate at room temperature for 15 min. Transfer 2-3 mL to the GeneXpert Cartridge and then load the cartridge into the instrument. Once loaded inside the GeneXpert Instrument, DNA extraction, amplification and detection steps occur automatically and the user is provided with a printable test results

Figure 4: GeneXpert MTB/RIF Assay Procedure [Ref. Boehme et al , NEJM 2010]<sup>[62]</sup> Add two volumes of sample treatment reagent to each volume of sputum. Mix well, incubate at room temperature for 15 min. Transfer 2-3 mL to the GeneXpert Cartridge and then load the cartridge into the instrument. Once loaded inside the GeneXpert Instrument, DNA extraction, amplification and detection steps occur automatically and the user is provided with a printable test results