Figure 3: Tarantula cubensis venom induces apoptosis in cancer cells. MCF7, HN5 and HEK293 cells were treated with 100 μμg/ml of Theranekron® (Tn) or same volume of ethanol (Et) for 24 h. (A) Western blot protein level of full length (35 kDa) and cleaved (17 and 19 kDa) caspase-3 in the cells. Increased level of cleaved caspase-3 in Theranekron® treated cells. (B) Fold change of ELISA active caspase-3 in the cells in the result of treatment with Theranekron®. MCF7 and HN5 cells showed higher level of increased active caspase-3in comparison with HEK293 cells. ** p<0.01, *** p<0.001, ns = Not Significant.